Composite
Pre A4

Part:BBa_K4060103:Design

Designed by: CHEN YAN-ZHEN   Group: iGEM21_NYCU-Taipei   (2021-09-30)


[Pre A4] a pBad ligated before J23106


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 145
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.

Source

The parts are from 2019 igem kits.

References